The establishment of vascular cell differentiation system from human ES/iPS cells and its application for the clarification of vascular pathology

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860557
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Kyoto University
• Principal Investigator: Taura Daisuke 京都大学, 医学(系)研究科(研究院), 助教 (10558612)
• Research Collaborator: Masakatsu Sone
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 血管細胞誘導 / iPS細胞

Outline of Final Research Achievements

Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have the potential to serve as a tool to investigate human development/differentiation. We modified our vascular differentiation system of human ES/iPS cells and we have induced vascular cells more effectively and stably from various ES/iPS cell clones without any feeder cells.
Using the iPS cells from patients with genetic abnormalities (patient-specific iPS cells) in vessels, we can observe vascular differentiation/development in vitro and identify new susceptibility genes associated with vascular abnormalities. Applying our differentiation system for Moyamoya-iPS, ADPKD-iPS and Takayasu-iPS cells, we have identified several molecules whose expression levels were upregulated or downregulated in vascular cells differentiated from patient-specific-iPSCs as compared to those from normal Japanese iPSCs. These results would contribute to a novel understanding of vascular abnormalities in each disease.

Free Research Field

再生医学