2015 Fiscal Year Final Research Report
Conformational change in transfer RNA is an early indicator of acute cellular damage
| Project/Area Number |
26860624
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | tRNA / 転移RNA / 急性腎障害 / 修飾核酸 / 1-メチルアデノシン / シュードウリジン / 酸化ストレス / バイオマーカー |
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| Outline of Final Research Achievements |
We revealed that the detection of 1-methyladenosine, which is a modified nucleoside containing in transfer RNA, is a useful new biomarker for diagnosis of acute kidney injury (Mishima et al. JASN, 2014). In addition, we showed that circulating 1-methyladenosine levels are significantly associated with the mortality in the general population using the sample of Ohasama cohort study. Therefore, tRNA damage reflects early oxidative stress damage, and detection of tRNA damage may be a useful tool for identifying organ damage and forming a clinical prognosis. Furthermore, we developed a method for detecting RNA modifications called immuno-northern blotting analysis and confirmed its various capabilities (Mishima et al. PLoS ONE, 2015).
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| Free Research Field |
腎臓病学
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