Analysis of kidney-specific transcriptional regulation of Epo gene and establishment of mouse model of renal anemia

2016 Fiscal Year Final Research Report

• Project/Area Number: 26860625
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Tohoku University
• Principal Investigator: Hirano Ikuo 東北大学, 医学系研究科, 助教 (00708117)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: エリスロポエチン / 低酸素ストレス / 転写調節機構

Outline of Final Research Achievements

We performed transgenic reporter mouse analyses to analyze a mechanism of renal Epo gene regulation. We have found that a 13.8-kbp region (CURE region), spanning the region from 17.4 kb to 3.6 kb upstream of the Epo gene, is necessary for renal Epo gene regulation. we have also found that mice lacking the CURE region suffer from severe anemia caused by the EPO-deficiency. We named the anemic mice “AnRED (anemic model with renal EPO deficiency) mice”.
CURE region harbors several phylogenetically conserved elements between humans and mice (among mammals). Our results obtained by transgenic reporter mouse analyses show that these elements redundantly and cooperatively work for the regulation of Epo gene in renal EPO-producing cells.

Free Research Field

分子生物学