The novel mechanism of aldosterone production in aldosterone-producing adenoma

2016 Fiscal Year Final Research Report

• Project/Area Number: 26860707
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Hiroshima University
• Principal Investigator: OKI KENJI 広島大学, 病院(医), 病院助教 (30638995)
• Research Collaborator: Gomez-Sanchez Celso E. University of Mississippi Medical Center
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 原発性アルドステロン症 / アルドステロン / G蛋白共役型受容体

Outline of Final Research Achievements

Microarray analysis targeting GPCR-associated genes was conducted using APA without known mutations (APA-WT) samples and APA with the KCNJ5 mutation (APA-KCNJ5). Gonadotropin-releasing hormone receptor (GNRHR) was the highest expression in APA-WT by microarray analysis. The quantitative polymerase chain reaction (qPCR) assay results revealed higher GNRHR expression levels in APA-WT samples those in APA-KCNJ5 samples. Patients with APA-WT (n=9), which showed higher GNRHR and LHCGR levels, had significantly higher GnRH-stimulated aldosterone response than those with APA-KCNJ5 (n=13) (P<0.05). HAC15 cells with KCNJ5 gene carrying T158A mutation exhibited a significantly lower GNRHR expression than that in control cells. We clarified increased expression of GNRHR and LHCGR in APA-WT, and the molecular analysis including the receptor expression associated with clinical findings of GnRH stimulation.

Free Research Field

医歯薬学