The role of U2AF1 mutations in the hematopoietic system

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860730
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Kyoto University
• Principal Investigator: Kataoka Keisuke 京都大学, 医学(系)研究科(研究院), 特定助教 (90631383)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 骨髄異形成症候群 / 急性骨髄性白血病 / 遺伝子改変マウス / U2AF1

Outline of Final Research Achievements

To examine the role of U2AF1 S34F mutations, which are frequently observed in myelodysplastic syndrome, I analyzed U2af1 S34F mutation conditional knock-in mice. At first, I created a conditional knock-in mouse model using FLEX switch system. After being crossed with Vav1-cre or Mx-cre mice, this mouse model did not show sufficient Cre-mediated recombination nor expression of U2af1-mutated allele. In addition, I did not find any differences in phenotypes of hematopoietic cells between wild-type and knock-in mice. Therefore, next, I created another conditional knock-in mouse model harboring loxP-STOP cassette-loxP U2af1 S34F allele. These mice are currently under investigation.

Free Research Field

血液内科学