Development of novel mesothelin-specific immunotherapy for treating pediatric refractory acute myeloid leukemia

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860790
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Shinshu University
• Principal Investigator: YANAGISAWA Ryu 信州大学, 医学部附属病院, 特任研究員 (80532043)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: メソセリン / 細胞傷害性Ｔ細胞 / 免疫療法 / 樹状細胞

Outline of Final Research Achievements

We attempted to generate mesothelin-specific cytotoxic T lymphocytes (CTLs) for developing novel immunotherapy for treating refractory pediatric acute myeloid leukemia. We cultured monocytes from peripheral blood mononuclear cells in a cytokine mix for 1 week so that monocytes could differentiate into dendritic cells. Furthermore, we attempted to generate mesothelin-specific CTLs after stimulating T lymphocytes with mesothelin-peptide-pulsed dendritic cells and a cytokine mix. Although we were unable to effectively generate mesothelin-specific CTLs, we found variable specificity in the generated mesothelin-specific CTLs. We believe our method needs further improvements before it can be applied clinically.

Free Research Field

小児血液腫瘍学