The role of microRNAs in the pathogenesis of dermatofibrosarcoma protuberans.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860886
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kumamoto University
• Principal Investigator: Kajihara Ikko 熊本大学, 大学院生命科学研究部, 特任助教 (90433036)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 隆起性皮膚線維肉腫 / miR-205 / LRP-1

Outline of Final Research Achievements

In this study, we investigated the role of microRNAs in the pathogenesis of dermatofibrosacoma protuberans (DFSP). MicroRNA PCR array showed the expression of miR-205 was down-regulated in DFSP compared with dermatofibroma and normal skin. miR-205 inhibitor increased cell proliferation and low-density lipoprotein receptorrelated protein-1 (LRP-1) protein in cultured normal dermal fibroblasts. Knockdown of LRP-1 suppressed cell growth and down-regulated extracellular signal-regulated kinase (ERK) phosphorylation in cultured DFSP cells. Taken together, LRP-1 overexpression caused by the miR-205 down-regulation may play a role in the abnormal proliferation of DFSP cells via directly regulating ERK phosphorylation.

Free Research Field

皮膚科学