Exome sequencing of human breast cancer tissues resistant to taxanes.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Nagoya City University
• Principal Investigator: Endo Yumi 名古屋市立大学, 医学(系)研究科(研究院), 助教 (20566228)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 乳腺外科学

Outline of Final Research Achievements

The aim of this study is to investigate the mechanisms of taxane resistance using whole exon sequencing and expression analyses in human breast cancer tissues. First we chose six breast cancer patients whose tumors responded well to anthracycline treatment but suffered disease progression on taxane treatment. Next, we examined whole exon sequencing on these samples. As a result, we found some somatic mutations which were considered to be candidate genes involved in the resistance to taxanes. Then, we performed mRNA expression analyses of these candidate genes in 122 breast cancers treated with taxanes. We correlated mRNA expression levels of these genes with clinicopathological factors and prognosis.
We identified 9 mutations common to all 6 patients analyzed in this study, and a further 16 mutations shared by 5 of them. Kaplan-Meier analyses showed that high level mRNA expression of 3 of these 25 genes was significantly associated with poorer disease-free survival.

Free Research Field

乳腺外科