2015 Fiscal Year Final Research Report
Evaluation of MAGE-D4 expression and functions in hepatocellular carcinoma
Project/Area Number |
26861066
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Nagoya University |
Principal Investigator |
TAKAMI Hideki 名古屋大学, 医学部附属病院, 病院助教 (40723028)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Keywords | 肝細胞癌 / MAGE-D4 / 血清マーカー |
Outline of Final Research Achievements |
Expression and functions of melanoma-associated antigen family D4 (MAGE-D4) was evaluated to identify novel molecular target of hepatocellular carcinoma (HCC). Five out of nine HCC cell lines, MAGE-D4 was remarkably overexpressed compared to control normal liver tissues. Knockdown of MAGE-D4 mediated by siRNA led to reduced migration and invasion abilities of HCC cells. No difference in the level of MAGE-D4 mRNA was found between three types of background liver (normal liver, chronic hepatitis and cirrhosis). MAGE-D4 protein was detected by immunohistochemical staining. Patients with high MAGE-D4 in HCC tissues had a significantly shorter survival. Expression of intra-tumoral MAGE-D4 had a correlation with VEGF, but not CD34 and HIF-1a. Some patients showed elevated serum MAGE-D4, however there were no correlations with AFP and PIVKA-II levels. MAGE-D4 may be a promising biomarker and therapeutic target of HCC.
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Free Research Field |
消化器外科学
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