Prevention of reperfusion injury and hemorrhagic infarction after acute recanalization threpy for cerebral infarction using TEMPO-RNP

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861139
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: University of Tsukuba
• Principal Investigator: MARUSHIMA Aiki 筑波大学, 医学医療系, 講師 (40722525)
• Research Collaborator: NAGASAKI Yukio 筑波大学, 数理物質科学研究科, 教授 ; HIRAYAMA Aki 筑波技術大学保健科学部, 東西医学統合医療センター, 教授 ; TSURUSHIMA Hideo 筑波大学, 医学医療系, 准教授 ; TSURUTA Wataro 虎ノ門病院, 脳神経血管内治療科 ; SUZUKI Kensuke 獨協医科大学, 越谷病院医学部, 教授 ; MATSUMARU Yuji 筑波大学, 医学医療系, 教授 ; YAMAMOTO Tetsuya 筑波大学, 医学医療系, 准教授 ; 松村 明 筑波大学, 医学医療系, 副学長 ; PUENTES Sandra 筑波大学, システム情報系, 助教 ; MATSUI Fumihiro 筑波大学, 医学医療系, 講師 ; HOSOO Hisayuki 筑波大学, 人間総合科学研究科 ; MUJAGIC Arnela 筑波大学, 人間総合科学研究科 ; ITO Hiromu 筑波大学, 人間総合科学研究科
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 脳虚血再灌流障害 / 脳動脈閉塞症 / 脳梗塞 / 脳神経血管ユニット保護 / RNP / ナノ粒子 / フリーラジカル消去剤 / 再開通療法

Outline of Final Research Achievements

Acute recanalization therapy is an important treatment for cerebral artery occlusion, but cerebral ischemia-reperfusion injury could cause a deterioration of cerebral infarction. We investigated neuro-endothelial unit protection effect of TEMPO-RNP (nitroxide radical-containing nanoparticles) for cerebral reperfusion injury in mice. Transient MCAO model mice received RNP or PBS through the common carotid artery. We revealed that intra-arterial TEMPO-RNP treatment decreased cerebral infarct size, protected neuronal cells and the BBB from reperfusion injury. RNP was detected on the endothelial cells and around the neuronal cells in the ischemic area. These results suggested that RNP could permeate the BBB at the first pass, remain in the ischemic area, and show ROS scavenging and neuro-endothelial unit protection effectively when injected intra-arterially.

Free Research Field

脳神経外科学