2016 Fiscal Year Final Research Report
Statin ameliorates lipopolysaccharide-induced dysfunction of blood-brain barrier
Project/Area Number |
26861156
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Neurosurgery
|
Research Institution | Nagasaki University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 血液脳関門 / スタチン / 脳虚血 |
Outline of Final Research Achievements |
Statins have a neuroprotective effect in neurological diseases, a pleiotropic effect possibly related to blood-brain barrier (BBB) function. In this study, we investigated the effects of statin on lipopolysaccharide (LPS)-induced BBB dysfunction in in vitro BBB model comprising co-culture of primary mouse brain endothelial cells, pericytes, and astrocytes. LPS (1 ng/ml, 24 hr) induced the impairment of permeability and transendothelial electrical resistance (TEER). Co-administration of statin alleviated the deleterious effects of LPS on BBB permeability and TEER. LPS increased IL-6, G-CSF, KC, MCP-1, RANTES release from our BBB model. Statin inhibited all these LPS-induced cytokine production. These data suggest that pitavastatin could ameliorate LPS-induced BBB dysfunction, which might be mediated by inhibiting LPS-induced cytokine production. Our present finding sheds light on the pharmacological significance of statins in various kinds of CNS diseases.
|
Free Research Field |
脳神経外科学、血液脳関門学
|