2016 Fiscal Year Final Research Report
Laryngeal Regeneration with Human-Induced Pluripotent Stem Cells
| Project/Area Number |
26861404
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | ヒトiPS細胞 / 上皮分化誘導 / 気道再建 |
|---|
| Outline of Final Research Achievements |
Immunofluorescence revealed the presence of pancytokeratin, epithelium associated proteins in iPS cells EGF and in iPS Vocal Fold Fibroblast(VFF) cultures. Transcript expression levels of CK14 was significantly increased for iPS cells VFF cultures only and measured concomitantly with cell morphology that was clearly cohesive and displaying a degree of nuclear polarity, suggestive of epithelial differentiation. iPS cells cultivated in 3D hydrogel with VFF demonstrated the most robust conversion evidence of epithelial differentiation
Presence of hydrogel was confirmed in vocal folds one and two weeks post injection. Histological results demonstrated that vocal folds injected with hydrogel scaffold containing EGF demonstrated less fibrosis than those with hydrogel only. iPS cells survived in injured rat vocal folds. hydrogel with iPS cells and EGF ameliorated the fibrotic response.
|
| Free Research Field |
耳鼻咽喉科学
|
