Acquired phenotype of myeloid cells under retinal pigment epithelium mediated ocular condition

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861438
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Horie Shintaro 東京医科歯科大学, 医学部附属病院, 助教 (40376744)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ミエロイド細胞 / マクロファージ / 網膜色素上皮細胞 / 糖尿病網膜症 / 眼内環境

Outline of Final Research Achievements

Human monocyte cell line THP-1 was stimulated by PMA (Phorbol 12-myristate 13-acetate). PMA-stimulated THP-1 was used as differentiated macrophages in vitro. PMA-THP-1 cells were cultured with AGEs or various cytokines (M1 inducer: IFN-γ, M2 inducer: IL-4 & IL-13, RPE secreting cytokine: PGE2). ELISA or FACS was performed in order to measure cytokines and chemokines in the cell supernatants. Quantitative mRNA expressions were also examined by RT-PCR. VEGF was highly secreted by PMA-stimulated THP-1 in the presence of AGEs or PGE2. Furthermore, not only massive secretion of IL-8 but also multiple increased productions of TNF-α, IL-1β, IL-6, IL-10, MCP-1, RANTES were induced by AGEs. In the presence of AGEs or PGE2, VEGF expression from macrophages is elevated. Furthermore, AGEs induces robust proinflammatory cytokines or chemokines productions, which indicates critical macrophage mediated ocular pathology triggered by AGEs.

Free Research Field

眼炎症