Elucidation of the optimal platelet-rich plasma for bone regeneration of a combination of stem cells and platelet-rich plasma

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861514
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: TAJIMA SATOSHI 順天堂大学, 医学部, 非常勤助教 (80724377)
• Research Collaborator: TOBITA Morikuni 順天堂大学, 医学部, 非常勤講師 (10599038) ; MIZUNO Hiroshi 順天堂大学, 医学部, 教授 (80343606)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 脂肪組織幹細胞 / 多血小板血漿 / 骨再生

Outline of Final Research Achievements

Adipose-derived stem cells (ASCs) have the potential to differentiate into bone. Platelet-rich plasma (PRP) is an interesting biological means to repair tissue by inducing chemotactic, proliferative, and anabolic cellular responses. This study evaluated bone regeneration using a combination of ASCs and PRP in a rat calvarial defect model. The in vitro study showed that the levels of growth factors secreted by ASCs were significantly increased by the addition of PRP. Transplantation of the ASC/PRP admixture had dramatic effects on bone regeneration overtime in comparison with rats that received other transplants. Furthermore, some ASCs directly differentiated into osteogenic cells in vivo. These findings suggest that the combination of ASCs and PRP has augmentative effects on bone regeneration. The ASC/PRP admixture may be a promising source for the clinical treatment of cranial defects.

Free Research Field

再生医療