Modulation of osteoblastic differentiation by G9a and ESET

2017 Fiscal Year Final Research Report

• Project/Area Number: 26861562
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Tsurumi University
• Principal Investigator: Ideno Hisashi 鶴見大学, 歯学部, 学部助手 (40435699)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: エピジェネティクス / ヒストンメチル化 / 骨芽細胞

Outline of Final Research Achievements

The purpose of this study was to elucidate role of G9a and ESET, both histone methyltransferases, during osteoblastic differentiation. Especially we focused on how they regulate the function of Runx2, which is a critical transcription factor for osteoblastic differentiation. We found that G9a and ESET enhanced transcriptional activity of Runx2. In G9a-knockout osteoblasts, we observed decreased expression of osteocalcin mRNA,　while expression of Runx2 was not affected. Moreover, we found that the C-terminal region of G9a was necessary for binding with Runx2 and enhancement its transcription activation. These results suggest that G9a is required for proper function of Runx2 during osteoblastic differentiation.

Free Research Field

基礎歯科学