Development of a new mouse model caused aneuploidy

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861585
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: Tanaka Hiroki 愛知県がんセンター(研究所), 腫瘍医化学部, リサーチレジデント (20725452)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 染色体異数性

Outline of Final Research Achievements

The aim of this study is the clarification of the cell fate of aneuploid cells and the development of a new cancer mouse model. We reported previously that the vimentin mutant mice display tetraploidy or aneuploidy resulting from cytokinetic failure and lens cataract. The fate of tetraploid cells remains largely unknown. We analyze the ability to repair wounds in the skin of the mutant mice. Early into wound healing, subcutaneous fibroblasts appeared binucleate tetraploid cells. Disappearance of tetraploidy coincided with an increase in aneuploidy. Thereafter, senescence-related markers were significantly elevated in mutant mice. our data suggest that following cytokinetic failure, a subset of tetraploid cells enters a new cell cycle and develops into aneuploid cells in vivo, which promote premature aging. We produced a new model mouse that displays cytokinesis failure induced aneuploidy in cells expressing desmin protein.

Free Research Field

腫瘍学