Detection of salivary novel biomarkers for diagnosis of oral cancer by metabolomics analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861726
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: Iwamoto Kazuki 愛媛大学, 医学部附属病院, 医員 (90723833)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 口腔癌 / メタボローム解析 / 唾液

Outline of Final Research Achievements

In this study, we have attempted to estimate the capability of metabolomics approach for detection of oral squamous cell carcinoma (OSCC) using saliva from patients. Saliva samples were obtained from 70 OSCC patients and 28 healthy adult volunteers. Capillary electrophoresis-time-of-flight mass spectrometry was employed for comprehensive analysis of metabolites. The area under the curve (AUC) more than 0.50 between OSCC patients and healthy volunteers were observed in 27 metabolites. Butanoate showed the highest AUC (0.6428) and statistically significant difference for the patients with OSCC compared with healthy volunteers. Moreover analysis for multiple metabolites improved diagnostic accuracy more than for a single metabolite. These results suggested that OSCC specific salivary metabolic profiles may be a useful diagnostic tool for the patients with OSCC.

Free Research Field

口腔癌の新規診断および治療法の開発