2015 Fiscal Year Final Research Report
imaging of sqaumous cell carcinoma cell with raman spectroscopy
| Project/Area Number |
26861765
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Fukui College of Health Sciences |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | ラマン分光法 / 扁平上皮癌細胞 / イメージング |
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| Outline of Final Research Achievements |
We analyzed cells of oral squamous cell carcinoma with raman spectroscopy to obtain clear cancer cell image. The serial transverse sections were prepared, one sample section was used for raman analysis, the other one was stained with H&E to confirm pathological structure. We could show the approximate form of cancer cell (about 10 micron square) with raman images reflecting area of some raman spectra peaks.
The image of raman spectroscopy indicates kinds and structures of molecule at semi-quantitatively. The raman images of cancer cells show the expression and distribution of molecule and its structure which belong to individual peaks of raman spectrum. Raman analysis don’t have need to make any treatments before measurement, it was suggested that this method might be able to obtain the distribution of many components in cancer cells by one time measurement.
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| Free Research Field |
医歯薬学
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