Elucidating the function of promoter DNA methylation status on IL1B gene regulation in human articular chondrocytes

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870031
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science; Orthopaedic surgery
• Research Institution: Tohoku University
• Principal Investigator: Hashimoto Ko 東北大学, 大学病院, 助教 (00718497)
• Research Collaborator: AKI Takashi ; OGASAWARA Masanori
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 変形性関節症 / 遺伝子発現 / 転写因子 / 網羅的解析

Outline of Final Research Achievements

The gene expression of chondrocytes was exhaustively analyzed (24,460 genes), and compared between osteoarthritic (OA) and non-OA human femoral heads. In contrast to our previous study conducted with caucasian samples, the expression level of IL1B was not upregulated in Japanese OA chondrocytes. In addition to the previously reported 3 OA-related genes in Japanese population, our research newly revealed 19 potential OA-related genes. Moreover, the gene expression patterns of OA chondrocytes were assumed to be different between Japanese and caucasian populations.
The transcription factors, FOS, LEF1 and CREB3L4 were found to be potential transcriptional regulators of MMP13, a key factor of OA pathogenesis, via DNA methylation of its promoter.

Free Research Field

関節軟骨の分子生物学