2015 Fiscal Year Final Research Report
Investigation of the cell based therapy for primary lung dysfunction after lung transplantation using mesenchymal stem cell
| Project/Area Number |
26870042
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
Respiratory surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 肺移植 / 細胞治療 / 虚血再灌流障害 / 間葉系幹細胞 |
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| Outline of Final Research Achievements |
Primary graft dysfunction is a major cause of morbidity and mortality after lung transplantation. Mesenchymal stem cells (MSCs) have a suppress inflammation and immune responses. We examined whether MSCs attenuate ischemia-reperfusion injury in a mouse model of lung transplantation. Mouse lungs were preserved at 4°C for 18 h. Human MSCs were injected into the lung grafts, and orthotopic left lung transplantation was performed. The lung isografts were reperfused for 6 hours. Protein concentration and cell count in Bronchioalveolar lavage fluid were significantly lower in the MSC-administered grafts than in the PBS-administered controls. Concentrations of proinflammatory cytokines showed a decreasing trend in the MSC-administered grafts compared with the controls.Pretransplant administration of MSC may attenuate ischemia-reperfusion injury after prolonged cold ischemia in the mouse model of lung transplantation.
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| Free Research Field |
胸部外科
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