Mechanistic analysis of iPSC production using paused iPSC

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology; General medical chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: Nishimura Ken 筑波大学, 医学医療系, 助教 (80500610)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: Klf4 / iPS細胞 / Tcl1 / 発現量依存的

Outline of Final Research Achievements

We succeeded to produce paused iPSCs with different KLF4 expression levels remaining at distinct intermediate stages of reprogramming. Among the genes highly expressing in Klf4-high paused iPSC, Tcl1 related to induction of high pluripotency. The analyses of Klf4-dose dependent transcriptional regulation in paused iPSC indicated that Klf4 binds to Tcl1 promoter dose dependently and lead to histone modifications, resulting in the induction of Tcl1 expression and pluripotency.

Free Research Field

iPS細胞誘導におけるエピジェネティックな遺伝子発現調節機構の解析