2016 Fiscal Year Final Research Report
New biochemical methods for plant enzymatic activity
| Project/Area Number |
26870252
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
Biofunction/Bioprocess
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| Research Institution | Shizuoka University |
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Principal Investigator |
|
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | テルペン |
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| Outline of Final Research Achievements |
We constructed a new methods for biochemical identification of plant enzymes using Nicotiana benthamiana as heterologous expression platform. 1-Deoxy-D-xylulose 5-phosphate gene and monoterpene synthases from Camellia sinensis installed in Nicotiana benthamiana gave higher amount of monoterenoids rather than wild type Nicotiana benthamiana. This new methods would open the door to identification of plant terpene synthases without neither adding substrate candidates nor extracting enzymes.
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| Free Research Field |
植物化学
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