2016 Fiscal Year Final Research Report
Breeding process innovation by short-period-gene-stocking techniques in transgenic plant of tetraploid ruzigrass (Brachiaria ruziziensis).
| Project/Area Number |
26870453
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop production science
Science in genetics and breeding
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | DREB1A / Brachiaria ruziziensis / drought tolerance / Apomixis |
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| Outline of Final Research Achievements |
The dehydration-responsive element-binding proteins 1 (DREB1s)/C-repeat binding factors (CBFs) are transcription factors that regulate the expression of abiotic stress-responsive genes in an ABA-independent manner. Ruzigrass (Brachiaria ruziziensis) is an important forage grass, however, it doesn’t perform well under drought conditions. To address this problem, we transformed a sexual tetraploid ruzigrass line with a construct carrying the Arabidopsis DREB1A gene driven by the promoter of the rice stress-inducible gene Lip9. The DREB1A gene was detected by PCR and stable integration of multiple copies of DREB1A was confirmed by DNA gel blot analysis in five independent lines. Reverse transcription PCR revealed expression of DREB1A in the transgenic lines only under drought stress conditions. These results demonstrate that stress-inducible overexpression of DREB1A has the potential to enhance abiotic stress tolerance in ruzigrass.
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| Free Research Field |
草類遺伝資源利用学
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