Exploratory analyses of comprehensive and quantitative mutation profiling using cell-free DNA

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870509
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics; Tumor diagnostics
• Research Institution: Wakayama Medical University
• Principal Investigator: Akamatsu Hiroaki 和歌山県立医科大学, 医学部, 助教 (10646582)
• Co-Investigator(Renkei-kenkyūsha): KIKUCHI Takashi 和歌山県立医科大学, 内科学第3講座, 助教 (40649050) ; YAMAMOTO Nobuyuki 和歌山県立医科大学, 内科学第3講座, 教授 (60298966) ; KOH Yasuhiro 和歌山県立医科大学, 内科学第3講座, 講師 (80426519)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: リキッドバイオプシー / EGFR遺伝子変異

Outline of Final Research Achievements

To establish multiplex assay of EGFR mutation using digital PCR, we first develop highly sensitive assay with plasmid and cell-lines. Next, we compared multiplex assay with duplex assay. Sensitivity was highly correlated (R2 = 0.99) between these assays. Thus, we subsequently analyzed clinical samples (60 advanced lung cancer patients and 17 healthy volunteers). Compared with tumor tissues, multiplex assay using plasma samples demonstrated sensitivity of 66% and specificity of 100%, respectively. Of those, patients who had distant metastasis demonstrated higher sensitivity (81%). Thus, this population may be suitable to further clinical trial.

Free Research Field

臨床腫瘍学