Study on distribution of H. pylori co-cultured with amoeba in environments

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870567
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health; Parasitology (including sanitary zoology)
• Research Institution: Kyorin University
• Principal Investigator: Hojo Fuhito 杏林大学, 医学部, 助教 (40580569)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: Helicobacter pylori / Acanthamoeba castellanii / 共生 / 感染経路

Outline of Final Research Achievements

Viable H. pylori was detected in A. castellanii until 7 hours after infection, but 24 hours or later, no colonies were detected. In whole co-culture with amoeba, viable H. pylori was detected even at 24 hours after infection. Morphological analysis revealed that almost all bacteria formed coccoid in single-culture of H. pylori. However spiral-formed bacteria remained in co-culture with A. castellanii. Transcriptome analysis showed that there was significant difference in gene expressions between single-culture and co-culture, implying that H. pylori took advantage of A. castellanii to survive in environment.

Free Research Field

微生物学