2016 Fiscal Year Final Research Report
Basic study of a long non-coding RNA, H19, as a novel therapeutic target for pancreatic cancer
| Project/Area Number |
26870622
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
Digestive surgery
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Research Collaborator |
ISHIWATA Toshiyuki
MATSUDA Yoko
TAKAHASHI Kimimasa
MICHISHITA Masaki
YAMAMOTO Masami
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | H19 / long non-coding RNA / pancreatic cancer |
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| Outline of Final Research Achievements |
Previously, we established a novel cell line derived from lung metastatic nodules developing after injection of PANC-1 human pancreatic carcinoma cells into an immunodeficient mouse. A microarray analysis showed that enhanced expression of H19 in lung metastasis derived cells compared to parental cells. In situ hybridization showed that H19 was detected in 17% of invasive ductal carcinomas and that H19 expression positively correlated with higher histological grades. Overexpression of H19 in PANC-1 cells induced higher motility and sphere formation ability whereas H19 inhibition using RNA interference technique showed opposite results. Intravenous injection of H19 knockdown PANC-1 cells yielded marked suppression of metastasis in immunodeficient mice. These findings suggest that H19 plays an important role in pancreatic cancer metastasis and H19 is a novel therapeutic target for metastasis of pancreatic cancer.
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| Free Research Field |
腫瘍病理学、獣医病理学
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