Synergy of gene silencing of lipid conjugate siRNAs targeting multiple oncogenes

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870770
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry; Biomolecular chemistry
• Research Institution: Yasuda Women's University
• Principal Investigator: Kubo takanori 安田女子大学, 薬学部, 講師 (90435751)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: siRNA / バイオコンジュゲート / ジーンサイレンシング / 脂肪酸 / DDS

Outline of Final Research Achievements

In this study, siRNAs were conjugated with palmitic acid at the 5'-end of the sense strand to enhance RNAi efficacy. The palmitoly-siRNAs (C16-siRNAs) targeting VEGF and β-catenin gene were synthesized by our simple method with high yield. We performed in vivo gene silencing experiments on a liver-metastatic HT29Luc tumor mouse model. Cells of the line HT29Luc, which derived from human colon cancer cell line and that stably expresses the Firefly luciferase gene, were established from a liver-metastatic tumor of orthotropic implantation model. After systemic administration of C16-siRNAs mixed with Invivofectamine, the RNAi effect was evaluated by the use of an in vivo imaging. Excellent gene-silencing effect of C16-siRNAs was observed in a liver-metastatic HT29Luc tumor mouse model.

Free Research Field

核酸化学、生物有機化学