in vitro BBB reconstruction based on controlling engineered 4D microenvironment

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870904
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering; Nano/Microsystems
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Yanagawa Fumiki 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 産総研特別研究員 (50645877)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 創薬スクリーニング / バイオMEMS / マイクロプロセス工学 / 三次元培養 / 灌流培養

Outline of Final Research Achievements

To fabricate microengineered platforms for cell culturing, photocleavable crosslinkers (NHS-PC-4armPEG and DBCO-PC-4armPEG) were developed. The characterization of cell behavior on photodegradable hydrogels, which were composed of the photocleavable crosslinkers, was investigated. The culture was performed without any cell damages. Also, the microengineerd devices were fabricated using photolithography techniques to perform 3D perfusion culture. The perfusion culture was also achieved using the fabricated microdevice, To commercialize the microdevices, they would need to be redesigned to evaluate the biological function of micro-engineered tissue.

Free Research Field

組織工学