Development of method for human risk assesment of environmental chemicals using metabolomics

2015 Fiscal Year Final Research Report

• Project/Area Number: 26881003
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Chiba University
• Principal Investigator: Eguchi Akifumi 千葉大学, 予防医学センター, 特任助教 (70595826)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: メタボロミクス / 残留性有機汚染物質 / 環境化学 / 分析化学

Outline of Final Research Achievements

In this study, we determine the high-throughput analytical method of ionic metabolome in serum and urine, using LC-MS/MS. The experimental values for almost target metabolome agreed well with the threshold values (coefficient of variation < 30%), indicating that the developed method might be suitable for rapid screening analysis. After the method development, we determined the metabolome analysis in serum from mice exposed to brominated flame retardant (BFRs) to identify toxicological biomarkers that characterized the exposure effects. The exposed mice showed lower levels of long-chain acylcarnitines and citrate cycle-related metabolites, and higher levels of some amino acids, long-chain phospholipids, and short-chain acylcarnitines. The change of serum metabolome profiles might be related to energy metabolism in exposed mice.

Free Research Field

環境分析化学