2015 Fiscal Year Final Research Report
High-Throughput Proteome Analysis by Parallel CE-MS System
| Project/Area Number |
26888021
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
KAWAI Takayuki 国立研究開発法人理化学研究所, 生命システム研究センター, 基礎科学特別研究員 (60738962)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Keywords | キャピラリー電気泳動 / 質量分析 |
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| Outline of Final Research Achievements |
For efficient peptide enrichment, large-volume sample stacking and transient isotachophoresis were combined. Up to 1000-fold sensitivity increase was achieved without losing separation performance. As for the parallel CE-MS, lower flow rate than 500 nL/min is required, so that 250 pL/min ultra-low flow rate CE-MS system with good ESI signal was achieved by fabricating 5 μm ID capillary to 15 μm OD with HF etching.
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| Free Research Field |
分析化学
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