Selective elimination of tumorigenic stem cells using hybrid nanoparticles and its application to regenerative medicine

2015 Fiscal Year Final Research Report

• Project/Area Number: 26889060
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Sojo University
• Principal Investigator: KOMIZU Yuji 崇城大学, 生物生命学部, 助教 (80735829)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 形質転換細胞 / 人工細胞膜 / 肝芽細胞 / 肝幹細胞 / 再生医療 / がん化 / ナノ医療 / リポソーム

Outline of Final Research Achievements

We have investigated the application of hybrid liposomes (HL) to selective elimination of the tumorigenic stem cells during hepatoblasts induction process. (1)The ratio of hepatoblastic cell number to total cell number was investigated by flow cytometric analysis. About 40 % of the cells were induced to be hepatoblasts by the treatment of 1 mM SB for 8 days of culture.
(2) During induction process, the tumorigenic cells appeared and produced abnormal prothrombin (PIVKA-Ⅱ), which is a clinical marker for hepatoma, and also formed colonies in soft agar plate, which is a criteria for neoplastic cell transformation. On the other hand, by the treatment with 0.4 mM HL for 48 h during the induction process, the colonies formed in soft agar plate remarkably decreased less than those of the non-treated cells. These results suggest that the tumorigenic hepatic stem cells could be eliminated by the treatment with HL.

Free Research Field

化学工学