Functional screening for engulfment of apoptotic cells with next generation sequence

2014 Fiscal Year Final Research Report

• Project/Area Number: 26893120
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: TODA Satoshi 京都大学, 医学(系)研究科(研究院), 研究員 (20738835)
• Project Period (FY): 2014-08-29 – 2015-03-31
• Keywords: 貪食 / マクロファージ / アポトーシス

Outline of Final Research Achievements

Macrophages recognize phosphatidylserine exposed on apoptotic cells and nuclei expelled from erythroblasts to engulf them. To identify factors that promote the engulfment, I aim to establish a functional screening system for engulfment of apoptotic cells with next generation sequence. I introduced cDNA library of resident peritoneal macrophages into a suspension cell line, Ba/F3, which has no ability to engulf apoptotic cells. Then I sorted Ba/F3 cells which engulfed apoptotic cells. Ba/F3 cells which acquired engulfment ability were enriched and I read cDNA sequences integrated in the cells with next generation sequence. As a result, several genes which have an ability to promote engulfment of apoptotic cells were detected.

Free Research Field

分子生物学