Elucidating the molecular mechanism of transcytosis by SH3YL1

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893149
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kobe University
• Principal Investigator: Yamamoto Hikaru 神戸大学, 学内共同利用施設等, 研究員 (70737337)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 脂質結合タンパク質 / 細胞死 / ミトコンドリア

Outline of Final Research Achievements

SH3YL1 is an evolutionarily conserved protein which contains a lipid-binding region called the SYLF (SH3yl1, Ysc84, Lsb3 and plant FYVE) domain. This study is aimed to identify an intracellular organelle whose trafficking is mediated by SH3YL1. Using COS-1 cell derived from an African green monkey kidney epithelium, we performed a live imaging of GFP-tagged human SH3YL1. It was revealed that SH3YL1 was located to mitochondria just before cell death. The lipid binding site of the SYLF domain influenced the mitochondrial localization of SH3YL1. Further, the recruitment of SH3YL1 occurred prior to the loss of membrane potential in mitochondria, indicating its role in apoptosis caused by mitochondrial dysfunction.

Free Research Field

細胞生物学