Regulation mechanism of the expression of acid-base related genes in the intercalated cells of the collecting duct in the kidney

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893202
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Kumamoto University
• Principal Investigator: Izumi Yuichiro 熊本大学, 医学部附属病院, その他 (20736243)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 腎集合尿細管 / 酸塩基平衡 / ユビキチン-プロテアソーム系

Outline of Final Research Achievements

Accumulation of nonvolatile acid that taken from food causes metabolic acidosis. Acid is excreted into urine by the intercalated cells of the collecting duct of the kidney. Adrenocortical hormone, aldosterone, is a key molecule that regulates acid excretion in the intercalated cells. The effect of acidosis (low pH) on the intercalated cells is unclear. We applied transcriptional start site-sequencing to characterize low pH stimulated-genes in the IN-IC cells, a rat intercalated cell line. We found the increases of genes that involve in ubiquitin-proteasome-pathway. We confirmed by Western blot that ubiquitination of proteins is enhanced by low pH.　Metabolic acidosis might regulate acid secretion in the intercalated cells by activating ubiquitin-proteasome-pathway.

Free Research Field

腎臓生理学