Development and functional analysis of biomarkers and therapeutic targets of giant cell tumor of bone.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26893267
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Juntendo University
• Principal Investigator: OKUBO TAKETO 順天堂大学, 医学部, 助教 (90732884)
• Research Collaborator: Suehara Yoshiyuki 順天堂大学, 医学部, 准教授 (70509405) ; Saito Tsuyoshi 順天堂大学, 医学部, 准教授 (80439736) ; Mukaihara Kenta ; Akaike Keisuke ; Tanabe Yu ; Ishii Midori
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 骨軟部腫瘍 / 骨巨細胞腫 / プロテオミクス

Outline of Final Research Achievements

To verify the prediction abitities of patient's outcomes in GCTBs, the protein expressions of the GPX family (especially GPX1 and GPX3) and the gene alteration of TP53 gene were analyzed using the large validation cohort. The results of validations showed that the expression of GPX family and gene mutation of TP53 had statistical significant difference of prognosis in GCTBs. In order to establish the protein profiles of GCTBs and elucidate the biological characteristics of GCTBs, comprehensive protein expression analyses were performed using frozen surgical specimen of GCTBs and the identified protein profiles were analyzed according to clinico-pathological features including patient's outcome and drug sensitivities. We found the characteristic proteins which were associated with denosumab treatments and sensitivities in GCTBs which is a therapeutic agent for GCTB.

Free Research Field

骨軟部腫瘍