2015 Fiscal Year Final Research Report
A study to resolve the mechanism by which osteoblasts and osteocytes regulate the differentiation of mesenchymal stem cells
Project/Area Number |
26893286
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Morphological basic dentistry
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Research Institution | Nihon University |
Principal Investigator |
AKIYAMA Yuko 日本大学, 歯学部, ポスト・ドクトラル・フェロー (90735622)
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Research Collaborator |
SHIMIZU Noriyoshi
HONDA Masaki
WATANABE Nobukazu
MIKAMI Yoshikazu
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Project Period (FY) |
2014-08-29 – 2016-03-31
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Keywords | 骨芽細胞 / 間葉系幹細胞 / ギャップジャンクション |
Outline of Final Research Achievements |
An analytical study of cell-cell communications between murine osteoblast-like MLO-A5 cells and mesenchymal stem cell (MSCs)-like C3H10T1/2 cells was performed. The mRNA expression levels of several osteogenic transcription factors did not differ between the co-cultured and mono-cultured C3H10T1/2 cells, but those of ALP and BSP were approximately 400-fold higher in the co-cultured cells. Patch clamp and biocytin transfer assays revealed gap junction-mediated communication between co-cultured C3H10T1/2 and MLO-A5 cells. A gap junction inhibitor suppressed the increases in the ALP and BSP mRNA expressions in co-cultured C3H10T1/2 cells. Furthermore, the histone acetylation levels were higher in co-cultured 10T-GFP cells than mono-cultured 10T-GFP cells. These results suggest that osteoblasts and BMSCs associate via gap junctions, and that gap junction-mediated signaling induces histone acetylation that leads to elevated transcription of the genes encoding ALP and BSP in MSCs.
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Free Research Field |
細胞生物学
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