The investigation of the effects of periodontitis on aterosclerosis

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893303
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontology
• Research Institution: Aichi Gakuin University
• Principal Investigator: MIYABE Megumi 愛知学院大学, 歯学部, 講師 (70734929)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 動脈硬化 / 歯周病

Outline of Final Research Achievements

In this tsudy, we focused on periodontal disease that is a chronic inflammatory disease. We investigated the effects of periodontitis on aterosclerosis.
In the in vivo experiments, we ligated the maxillary second molar of the mouse with wire to cause periodontal disease, and then the mouse were subjected to wire injury operation of the left femoral artery. We were able to collect the samples of blood and/or tissue from the mouse. In the in vitro experiments, to investigate the effects of periodontitis on SMC growth at a mechanistic level, HASMCs were treated with LPS. Cell number was assessed by MTS-based assay. DNA synthesis was measured with a BrdU proliferation assay kit. A condition of the LPS which HASMC was easy to growth became clear.

Free Research Field

動脈硬化