1986 Fiscal Year Final Research Report Summary
Automated high-performance apparatus for quantitative immunoblot analysis: Manufacture trial and its application to cell biochemistry
| Project/Area Number |
59870011
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MURACHI Takashi Kyoto University, Faculty of Medicine, Professor, 医学部, 教授 (10089104)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASE Yuzo Tateisi Institute of Life Sciences, Laboratory Manager, ライフサイエンス研究所, 室長
KOBAYASHI Shigeki Tateisi Institute of Life Sciences, Director, ライフサイエンス研究所, 所長
KANNAGI Reiji Kyoto University, Faculty of Medicine, Lecturer, 医学部, 講師 (80161389)
TOTANI Masayuki Kyoto University, Faculty of Medicine, Lecturer, 医学部, 講師 (70163988)
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| Project Period (FY) |
1984 – 1986
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| Keywords | protein / qualitative analysis / microquantitative determination / densitometer / data processing / immunoblotting / multiple staining / 細胞内成分分析 |
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| Research Abstract |
1. Establishment of fundamental conditions for quantitative determination of immunoblots (1984). (1) A linear relationship in the range of 5 ng to 10 <mu> g of proteins was established using peroxidase. (2) High speed data processing enabled rapid scanning (3 cm/sec) and 1 M byte memory storage sufficed quantitative analysis.
2. Development of a testing machine and the fundamental experiment for applications to intracellular analysis (1985). Concentration vs. absorbance was linear over the range of optical density of 0 to 2.2 and the best sensitivity was obtained.
3. The completion of a testing machine and practical studies on intracellular analysis (1986). Biochemical and immunohistochemical distribution studies in various tissues with regard to calpain and calpastatin were advanced, and quantitative variation of these proteins was estimated by immunoblotting.
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