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1987 Fiscal Year Final Research Report Summary

Investigation of human genetic marker and human specific component in body fluids and its application to forensic medicine

Research Project

Project/Area Number 60304057
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionAkita University

Principal Investigator

YOSHIOKA Naofumi  Assoc.Prof. of Forensic Med, Akita Univ. School of Med., 医学部, 助教授 (80108935)

Co-Investigator(Kenkyū-buntansha) ISHIMOTO Gouishi  Assoc.Prof. of Forensic Med., Mie Univ. School of Med., 医学部, 助教授 (30024673)
豊増 翼  帝京大学, 文学部, 教授
FUKUSHIMA Hirofumi  Assoc.Prof. of Forensic Med., Shinshu Univ. School of Med., 医学部, 助教授 (70135218)
HARADA Katsuji  Assoc.Prof. of Forensic Med., Tsukuba Univ. Community Med., 社会医学系, 助教授 (60086618)
SAGISAKA Kaoru  Prof. of Forensic med., Tohoku Univ. School fo Med., 医学部, 教授 (70006740)
TOYOMASU Tasuku  Prof. of Teikyo Univ. Institute of Litarature
Project Period (FY) 1985 – 1987
KeywordsBlood Group / Blood Group Substance / Species Indentification / Immunoassay / Electrophoresis / Genetic Marker / Polymorphism / 免疫化学
Research Abstract

Total sixteen workers joined and worked out this big research project concerning with their own specialized field such as red blood cell, white blood cell, serum protein,seminal fluid, salivary protein or sweat. Some of works have reached the goal and have improved evidently to be adapted for forensic medicine practically. Here, actual results are given; 1) Specific protein in sweat was proved and its own antibody was produced by immunizing rabbits. This research can contribute for identifying sweat from its stain or mixed stain with other body fluid. 2) Genetic systems or gene frequencies of serum orsomucoid, glutathione S-transferase (GST) and saliva aldehyde dehydrogenase (ALDH) were established and physiological back ground of polymorphism of GST and ALDH was studied with the samples from alcoholics. These findings can be adapted for paternity test and personal indentification in the field of forensic medicine, and for clinical research to find relation to alcoholics. 3) The progre … More ss method to indentify species (especially human) on hemoglobin, salicary protein and some kinds of serum protein as a human specific marker was woeked out. This basic research is very important for forensic field and may be considered for practical usage. 4) Serological difference of H substance between red blood cell and in saliva, and its distribution in salivary gland were clarified. Quantitative investigation of Lewis a substance and radiometric procedure for blood typing in addition to the work of H substance can contribute not only to forensic medicine but also to clinical research project. 5) Population study of some kinds of genetic markers, immunochemical analysis of serum proteins and enzyme linked immuno sorbent assay for organ specific enzyme were performed. Some of them are consifered to be continued for further progressing study to adapt practically. As mentionedabove, This project could get a lot of good results for forensic medicine, but, by taking recent quick progress in genetics, anthropology and immunology into careful consideration, it is recommeded that this kind of research project should be continuously kept up with introducing the new est technology and knowledge by systemic research group. Less

  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] 高取 健彦: 法中毒学ニュース. 4. 94-105 (1986)

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  • [Publications] Takatori, K: Act. Crim. Japon.IN PRESS.

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  • [Publications] Yoshioka, N: Biochem. J.234. 457-461 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] 進藤祥子: 法医学の実際と研究. 29. 13-17 (1986)

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  • [Publications] 新津 ひさえ: 生物物理化学. 29. 83-88 (1985)

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      「研究成果報告書概要(和文)」より
  • [Publications] 常盤和雄: 日本法医学雑誌. 41. 102-108 (1987)

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      「研究成果報告書概要(和文)」より
  • [Publications] 原田勝二: 日本法医学雑誌. IN PRESS.

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  • [Publications] Toyomasu, T.: Human Heredity. 37. 124-126 (1987)

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      「研究成果報告書概要(和文)」より
  • [Publications] Fukushima, H.: Jpn. J. Legal Med.,. 40. 101-104 (1986)

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  • [Publications] Fukushima, H.: Act.Crim.Japon.53. 149-152 (1987)

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  • [Publications] Iwasa, M.: Tohoku J. exp. Ned.145. 283-290 (1985)

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  • [Publications] 大谷 勲: 法医学の実際と研究. 28. 5-8 (1985)

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  • [Publications] Sagisaka, K.: Act. Crim. Jpn.51. 80-83 (1985)

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  • [Publications] Sagisaka, K.: Act. Crim.Jpn.53. 58-62 (1987)

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  • [Publications] 工藤敏行: 法医学の実際と研究. 30. 17-19 (1987)

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  • [Publications] 梅津和夫: 生物物理化学. 29. 381-384 (1985)

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  • [Publications] Unetsu, K.: Elctrophoresis. 8. 333-334 (1987)

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      「研究成果報告書概要(和文)」より
  • [Publications] Harada, S.: Hum. Genet.71. 560-565 (1987)

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  • [Publications] Ishimoto, G.: Adv. Forens. Haemogenet.1. 190-191 (1986)

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  • [Publications] Ishimoto, G.: J. Anthrop. Soc. Nippon. 95. 515-518 (1987)

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  • [Publications] 大島美奈子: 日本法医学雑誌. 40. 287-292 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] 大島美奈子: 日本法医学雑誌. 41. 210-216 (1987)

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  • [Publications] Ogata, M.: J. Forensic Sci. 32. IN PRESS (1987)

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  • [Publications] T. Takatori: "Lewis typing of human bloodstains by enzyme linked immunosorbent assay (ELISA) using monoclonal anti-Le^a and anti-Le^b" Journal of Forensic Science. 32. 900-905 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] N. Yoshioka: "Antigenic structure of human haemoglobin" Biochemical Journal. 234. 441-447 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] N. Yoshioka: "Haemoglobin binding with haptoglobin-Localization of the haptoglobin binding stres on the beta chain of human haemolobin by synthetic overlapping peptides encompassing the entire chain." Biochemical Journal. 234. 453-456 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Sagisaka: "Further study of new secretor status of human saliva" Acta Criminologiae et Medicinae Legalis Japomica. 51. 80-83 (1985)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Sagisaka: "Availability of anti-II sera specific for red cell of saliva in blood grouping of body fluid stains" Acta Criminologiae et Medicinae Legalis Japomica. 53. 58-62 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K.Umetsu: "Orsomucoid typing by print lectinofixation: A new technique for isolectin focusing. Two common allele in Japan" Human Jenetics. 71. 223-224 (1985)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Umetsu: "Alph I antitrypsin and orsomucoid phenotyping using allo A lectin peroxidasefor detection" Electrophoresis. 8. 333-334 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Harada: "Liver glutathione S-transferase polymorphism im Japanese and pharmacogenetic importance" Human Genetics. 71. 560-565 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Harada: "Aldehyde dehydrogenase and glutathione S-transferase polymorphism: Association between phenotype frequencies and alcoholism" Progress in Clinical and Biological Research. 241. 241-250 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] T. Toyomasu: "Genetic polymorphism of red cell glutamate-pyruvate-transaminase in Japanese" Human Heredity. 37. 124-126 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Fukushima: "Segregation of Rh1(D) and Rh25(LW) antigens of humanRh blood group from the triton-trated fetal erythrocyte membranes" Japanese Journal of Legal Medicine. 40. 101-104 (1986)

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  • [Publications] H. Fukushima: "Quantitative comparison of Rh1(D) antige on D, D^u and red cells by radioimmunoassay using ^<125>I-protein A" International Journal of Biochemistry. 18. 1147-1149 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M. Iwasa: "A new individual variation of saliva detected with rabbit anti-human saliva serum" Tohoku Journal of experimental Medicine. 145. 283-290 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I. Ohya: "Identification of human saliva by antisera to alpha-amylase in human salivary glands" Tohoku Journal of experimental Medicine. 150. 309-315 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] G. Ishimoto: "Distribution of serum paraoxon hydrolyzing activity in family and population samples" Japanese journal of Legal Medicine. 41. 347-350 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] G. Ishimoto: "A salivary protein polymorphism found in the extra-parotid secretion of macaques" Journal of Onthropology Society (Nippon). 95. 515-518 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M. Oshima: "Comparative immunochemical studies of human and animal bete-lipoprotein, alpha-2-macroglobulin and alpha-1-antitrypsin by ELISA methods" Japanese Journal of Legel Medicine. 41. 351-357 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M. Oshima: "Application of ELISA methods for the identification of pronase-digested human serum proteins" Japanese Journal of Legal Medicine. 41. 358-364 (1987)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M Ogata: "Detection of Le^a substance in saliva stains by enzyme-linked immunosorbent assay(ELISA) using anti gum-arabic serum" Journal of Forensic Science. 32. (1987)

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  • [Publications] K. Harada: Liver and Alcohol Genetic background of alcoholic related enzyme. Chugai-Igaku, 24-32 (1986)

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      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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