1988 Fiscal Year Final Research Report Summary
Structure and Molecular Mechanisms of Time controlling Genes
| Project/Area Number |
60440001
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
遺伝学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YANAGISAWA Kaichiro Institute of Biological Sciences, 生物科学系, 教授 (60015899)
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| Project Period (FY) |
1985 – 1988
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| Keywords | Cellular slime mold / life-span / time controlling gene / transformation / 発生時間 |
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| Research Abstract |
Life-span in organisms seems to be genetically regulated. In a series of experiments, attempts were made to clarify whether time-controlling genes really exist, and if it is, how they control life-span. We used cellular slime mold Dictyostelium discoideum, a Kind of soil amoebae, as a model system for the experiments. The amoeboid cells feed bacteria and proliferates by fission during growth stage. However, in developmental stage, they form multicellular fruiting-bodies. Process of development takes 24 hrs at 22 C. First, a number of mutants which produce fruiting-bodies within 16 hrs were isolated. 2) The mutants were genetically analized and found that rapid development was caused by recessive mutation of a single gene. 3) Biochemical works clarified that CAMP metabolisms during development was very much abnormal in these mutants. Attempts were then made to isolate the time-controlling gene to determine its structure and products. 4) A shuttle vector was constructed from slime mold plasmid pDG1 and E.coli plasmid pBR322 for gene cloning. 6) High frequent transformation method was established by modification of electroporation nethod.
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