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1987 Fiscal Year Final Research Report Summary

Micromanipulation systen for Mammalian Egg And Embryo By The Control of Personal Computer

Research Project

Project/Area Number 60480082
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionMEIJI UNIVERSITY

Principal Investigator

OGAWA Shyoso  Faculty of Africulture,Meiji University,Professor, 農学部, 教授 (00061797)

Co-Investigator(Kenkyū-buntansha) TOMODA Shinobu  Faculty of Agriculture, Meiji university Professor, 農学部, 教授 (40061676)
KANOU Yasuhiko  Faculty of Agriculture,Meiji University Professor, 農学部, 教授 (80011849)
Project Period (FY) 1985 – 1987
KeywordsCOMPUTER-CONTROLLED MICROSURGERY / EMBRYO SURGERY / SOPHISTICATED MICROMANIPULATION / 顕微切断 / マイクロコンピュレーター / マイクロマニピュレーション
Research Abstract

The microsurgeries of masmmalian eggs and early embryos were attempted using a three-dimensilnal oil pressure micromanipulator driven by pulse motors. The pulse signals that regulate the three pulse motors for the X,Y and Z axes were controlled by the personal computer programs. A gap sensor was attached to the working arm of the micromanipulators. Programs were produced so that when an error between a command from the personal computer and a movement of the micromanipulator was detected,a feedback mechanism allowed the personal computer to correct the error. Movements of the tips of the microtools were precisely regulated. For holding of embryos, a holding micromanipulator driven by pulse motor was produced. A pressure sensor was attached to the connecting base of the holding pipet, so that the feedback system could assure the stable holding.
The following basic and more sophisticated micromanipulations on mouse pronuclear ova and morulae and, in some cases, on bovine morulae were successful and ensured in most cases used; (1) introduction of the micropipet into the male pronucleus, (2) lateral bisection of the morulae, (3) cutting healthy portion out a partially collapsing bovine embryo, and (4) intra-zonal aggregation of two-half morulae. These results suggest that the feasibility of and potentials for further applications of such a personal computer-controlled micromanipulator.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] S. Ogawa;H. Nagashima 他: Theriogenology. 27. 298 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Ogawa;K. Shibata 他: Theriogenology. 27. 262 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Ogawa;H. Nagashima 他: Jpn. J. Anim. Reprod.33. 151-154 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Ogawa;K. Kashiwazaki 他: Exp. Anim.37.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 尾川昭三, 加納康彦 他: 家畜繁殖誌. 33. 140-145 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Ogawa;Y. Kanou 他: Bul. Fac. Agric, Meiju Univ.7. 1-8 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Ogawa, K.Shibata, et al.: "Development of half embryos produced from morulas and blastocysts in pigs." Theriogenology. 27. 298 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ogawa, H.Nagashima, et al.: "Microsurgical bisection of expanding blastocysts for sexing of pigembryos." Jpn.J.Anim.Reprod.33. 151-154 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ogawa, Y.Kanou, et al.: "Detection of fluorescent body in spermatozoa of bull,boar,dog,rat and mouse." Jpn.J.Anim.Reprod.33. 140-145 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ogawa, H.Nagashima, et al.: "Survival of pig hatched blastocysts exposed below 15゜C." Theriogenology. 29. 280 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ogawa, K.Kashiwazaki, et al.: "Sexing of half-embryo produced by microsurgical bisection of morular and transfer and production of two-sexed half-embryos chimaeras and homozygotic twin chimaeras in mice." Exp.Anim.37. (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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