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1987 Fiscal Year Final Research Report Summary

Studies on cytotoxic substances of Fusobacterium necrophorum

Research Project

Project/Area Number 60480087
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionFaculty of Agriculture

Principal Investigator

KANOE Masamitsu  Faculty of Agriculture, Yamaguchi University, Professor, 農学部, 教授 (00035099)

Co-Investigator(Kenkyū-buntansha) KAI Kazushige  Faculty of Agriculture, Yamaguchi University,Assoc,Prof., 農学部, 助教授 (60085628)
Project Period (FY) 1985 – 1987
KeywordsNecrose bacillus / Leukocidin / Cytotoxic substances / Fusobacterium necrophorum / フソバクテリウム / 溶血毒 / 感染防御抗原
Research Abstract

Partial purification and cytotoxic effects of Fusobacterium necrophorum leukocidin on bovine different cells were investigated.
F. necrophorum leukocidin in diffusate of dialysis culture was partially purified by ammonium sulfate saturation and by ultrafiltration. It was free from the bacterial hemolysin and additional toxic substances.
Scanning electron microscopy revealed diminution and loss of the microvilli and destruction of the leukocidin-treated ruminal and hepatic cells. Bovine peripheral granulocytes were completely destroyed by the toxin. In lymphocytes the leukocidin induced loss of microvilli and irregular shapes of the affected cells.
Bovine anti leukocidin was measured by the ELISA test using the leukocidin. These findings indicate that the leukocidin appeared to be associated with the virulence factors, playing role in the pathogenesis of necrobacillosis.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] KANOE Masamitsu et al.: Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene series A. 261. 170-176 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KANOE Masamitsu et al.: Microbios Letters. 35. 119-123 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鹿江雅光: 日本獣医師会雑誌. 40. 401-407 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ISHII Toshiaki et al.: Medical Microbiology and Immunology. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KANOE Masamitsu et al.: Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene zeries A. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KANOE Masamitsu et al.: "Partial characterization of leukocidin from Fusobacterium necrophorum" Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 261. 170-176 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KANOE Masamitsu et al.: "Effects of Fusobacterium necrophorum leukocidin on bovine ruminal cells" Microbios Letters. 35. 119-123 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KANOE Masamitsu: "Bovine necrobacillosis (in Japanese)" Journal of the Japan Veterinary Medical Association. 40. 401-407 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ISHII Toshiaki et al.: "Cytotoxic effects of a leukocidin from Fusobacterium necrophorum on bovine hepatic cells" Medical Microbiology and Immunology. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KANOE Masamitsu et al.: "Effects of leukocidin from Fusobacterium necrophorum on bovine peripheral leukocytes in vitro" Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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