Idendification of the glutamate receptor and analysis of ionchannel dynamics

1987 Fiscal Year Final Research Report Summary

• Project/Area Number: 60480118
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Tokyo Metropolitan Institute for Neurosciences
• Principal Investigator: KAWAI Nobufumi Tokyo Metropolitan Institute for Neurosciences, 病態生理, 副参事 (00073065)
• Co-Investigator(Kenkyū-buntansha): SHIMAZAKI Kuniko Tokyo Metropolitan Institute for Neuroscinces, 病態生理, 研究員 (40142153) ; MIWA Akikio Tokyo Metropolitan Institute for Neurociences, 病態生理, 研究員 (60142155) ; SAITO Mitsuyoshi Tokyo Metropolitan Institute for Neurosciences ; YOSHIOKA Masanori Setsunan University (30012652) ; PAN-HOU Hidemitsu Setsunan University
• Project Period (FY): 1985 – 1987
• Keywords: glutamate / glutamate receptor / synaptic transmission / spider toxin Joro spider toxin / JSTX / GlutamateB

Research Abstract

We have found that a spider ( Nephila Clavata, Joro spider) venom contains a potent inhibitor of the glutamate receptors. The toxin purified from the venom of Joro spider (JSTX) blocks both the excitatory postsynaptic potentials and glutamate-induced excitation in vertebrate as well as invertebrate synapses. Chemical characterization of JSTX revealed a unique structure containing 2,4-dihydroxyphenylacetylaspargine combined with various types of polyamines. Using synthetic compounds of the spider toxin and various type of analogs the structure-activity relationship of these compounds and blocking action was studied on the glutamate receptors. We have fond that 2,4 dihydroxyphenylacetyl-asparaginayl-cadaverine was essential for revealing the suppressive action on the glutamate receptor, whereas the remaining part containing various polyamine chains was responsible for binding of the toxin molecule to receptor-ionchannel complex.

Research Products

• [Publications] Saito, M.;Kawai, N.: Developmental Brain Research. 18. 97-102 (1985)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Saito, M.;Kawai, N.;Miwa, A.;Yamagishi, S.;Furuya, K.: Neuroscience Research. 2. 197-307 (1985)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Saito, M.;Kawai, N.: Developmental Biology. 121. 90-96 (1987)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miwa, A.;Kawai, N.;Saito, M.;Pan-Hou, H;Yoshioka, M.: Jourmal of Neurophysiology. 58. 319-326 (1987)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Aramaki, T.;Yasuhara, T.;Higashijima, T.;Miwa, A.;Kawai, N.;Nakajima, T.: Proceeding of Japan Academy. 63;SerB. 359-362 (1986)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miwa, A.;Kawai, N.;Ui, M.: Brain Research. 416. 162-165 (1987)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Aranaki, Y.,Yasuhara,T., Higashijima, T., Yoshioka, M., Miwa, Akawai, N and Nakajima, T.: "Chemical characterization of spider toxin, JSTX and NSTX." Proc. Japan Acad.62, Ser.B. 359-362 (1986)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Saito, M. and Kawai, N.: "Patch clamp study of single glutamate channel in the insect neruomuscular synapse." Developmental Biology. 121. 90-96 (1987)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miwa, A., Kawai, N. and Ui, M.: "Pertussis toxin blocks the presynaptic glutamate receptors - a novel " Glutamate_B" receptor in the lobster neuromuscular synapse" Brain Research. 416. 162-165 (1987)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miwa, A., Kawai, N., Saito, M., Pan-Hou,H. S. and Yoshioka, M.: "Effect of a spider toxin (JSTX) on excitatory postsynaptic current at neuromuscular synapse of spiny lobster." Journal of Neurophysiology. 58. 319-326 (1987)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shudo, K. Endo,Y.,Hashimoto, Y., Aramaki, Y., Nakajima, T. and Kawi, N.: "Newly synthesized analogues of the spider toxin blocksthe crustacean glutamate receptor." Neuroscience Research. 5. 82-85 (1987)
  • Description: 「研究成果報告書概要(欧文)」より