1987 Fiscal Year Final Research Report Summary

Structure and Function of RNA on the Surface of Tumor Cells

Research Project

Project/Area Number	60570167
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Experimental pathology
Research Institution	National Cancer Center Research Institute
Principal Investigator	TERASAKI Takeo National Cancer Center Research Institute, 病理部, 主任研究官 (80142652)
Project Period (FY)	1985 – 1987
Keywords	Cell surface / RNA / Tumor Cell / Structure / Electron Microscope / Anti-RNA Antibody / 表面荷電
Research Abstract	(A) RNAs on the cell surfaces of two nonleukemic and two leukemic strains of mouse ascites tumor cells were studied by fractionating the RNAs released from the cell surface by gentle pronase treatment after sucrose density gradient centrifugation, by indirect membrane immunofluorescence that used anti-rna antibody and by cell electrophoresis. Results suggested that RNA on the surfaces of nonleukemic ascites tumor cells is composed predominantly of 18s and 28s ribosomal RNA. (B) The structure of RNA on the surface of Ehrlich ascites tumor cells was studied under an electron microscope using both plasma polymerization replica films and ultra-thin sections of the cells. Results suggested that RNA on the surface of Ehrlich ascites tumor cell is present in the form of ribosome-like particles.

(6 results)