1986 Fiscal Year Final Research Report Summary
Evaluation of Elution Pattern of Serum and CSF Prolactin Fractionated by Gel Filtration Chromatography in Cases with Bromocriptine-resistant Prolactinoma
| Project/Area Number |
60570657
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Akita University |
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Principal Investigator |
SAKAMOTO Tetsuya Akita University, Associate Professor, 医学部, 助教授 (00004713)
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| Co-Investigator(Kenkyū-buntansha) |
WAKISAKA Akira Akita University, Associate Professor, 医学部, 助教授 (60006707)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Prolactinoma / Prolaction / Heterogeneity / Radioimmuno Assay / Radioreceptor Assay / RRA法 |
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| Research Abstract |
In order to study the effect of Bromocriptine on prolactinoma gel filtration chromatographic behavior of prolactin was measured in both serum and cerebrospinal fluid of six cases of individuals with prolactinomas, who showed high concentration of prolactin after surgery and administration of Bromocriptine.
Prolactin levels of paired blood and CSF samples were measured by using a double-antibody radioimmunoassay. Molecular weights of prolactin were estimated a ultrogel AcA 44 column with standard molecular markers.
Gel filtration of serum and CSF gave three types of prolactin with different molecular weights; "Little" "Big" and "Big-big". In postoperative serum samples, the proportion of three components were 71 <-!+> 4%, 19 <-!+> 2%, and 10 <-!+> 4%, respectively. In postoperative CSF samples, the proportion were 96 <-!+> 2%, 2 <-!+> 1%, 2 <-!+> 1%, respectively. "Little" prolactin was much less in proportion after administration of Bromocriptine.
These results indicate that prolactin molecule in tumor parenchym leaks into CSF, and that there would be different mechanism of Bromocriptine in reducing prolactin level in both serum and CSF among individuals cases. Large molecular prolactin may arise as a derivative or aggregates of standard prolactin.
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