1987 Fiscal Year Final Research Report Summary
ANALYSIS OF B CELL ACTIVATION AND DIFFERENTIATION IN PRIMARY HYPOGAMMAGLOBULINEMIA.
| Project/Area Number |
61480213
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | HOKKAIDO UNIVERSITY |
|---|
Principal Investigator |
MATSUMOTO Shuzo HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, 医学部, 教授 (80000933)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKEKOSHI Yasuo HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, 医学部, 助手 (50109427)
SAKIYAMA Yukio HOKKAIDO UNIVERSITY MEDICAL HOSPITAL, 医学部附属病院, 講師 (80133734)
TOGASHI Takehiro HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, 医学部, 講師 (70001930)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Keywords | Primary hypogammaglobulinemia / SAC / IL-2 / EBV / pre B cell / μ鎖mRNA |
|---|
| Research Abstract |
The activation and differentation of B cells in 13 patients with primary hypogammaglobulinemia were studied by using EBV induced lymphoblastoic cell lines (LCL) and stimulation with SAC and SAC and SAC+recombinant IL-2 (rIL-2). DNA rearrangement and mRNA were also studied in a case with the LCL of pre B cell phenotype.
I. LCL were established from peripheral blood lymphocytes with EBV in 7 cases, and the LCL from 6 cases produced predominantly IgM.
II. B cells from 7 patients were tested for their ability to proliferate and differentiate under stimulation of SAC and SAC+rIL-2. They showed three different types as follows: (1)no proliferation under stimulation of SAC or SAC+rIL-2, (2) normal proliferation to SAC but not to SAC+rIL-2, (3)normal proliferation to SAC and SAC+rLL-2, but no differentation to Ig secretory cells.
III. In the LCL of pre B cell phenotype, mRNA of <mu> chain showed to be short in either membranous of secretory types.
|
