1988 Fiscal Year Final Research Report Summary

Molecular study on the muscle protein, paramyosin gene of Caenorhabditis elegans

Research Project

Project/Area Number	61480477
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	分子遺伝学・分子生理学
Research Institution	Okayama University
Principal Investigator	KAGAWA HIROAKI Associate Professor, 理学部, 助教授 (10022732)
Project Period (FY)	1986 – 1987
Keywords	Caenorhabditis elegans / paramyosin / muscle protein / exon-expression cloning / monoclonal antibody / invertebrate / coiled-coil structure
Research Abstract	Nucleotide sequence of the paramyosin gene of Caenorhabditis elegans was determined by using shot gun procedure of Sanger method. The accession number in the EMBL/GenBak/DDBJ Nucleotide Sequence Databases was X08068. The gene was separated into 10 exons which encode 866 amino acid residues having molecular mass of 99,890 daltons. Amino acid sequence promised alpha-helical-coil-coil structure of the protein. Potential charge-mediated interactions between paramyosin and between paramyosin and myosinrod were caluculated using a model. Antigenic sites of the protein against antiparamyosin antiserum and three monoclonal antibodies were identified by immunological assay of fusion proteins which were produced in bacterial cells. The result could be useful for understanding molecular assembly of thick filament in invertebrates.