1987 Fiscal Year Final Research Report Summary
Cell-cell adhesion of the cellular slime molds and liposome-cell interactions
| Project/Area Number |
61540481
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | Dep. Botany, Fac. Science, Hokkaido Univ. |
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Principal Investigator |
OCHIAE Hiroshi Fac. of Science, Hokkaido Univ., 理学部, 助教授 (10002122)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Keizo Fac. of Pharmacology, Tokyo Univ., 薬学部, 教授 (30072937)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Cellular slime mold / Dictyostelium discoideum / Cell-cell adhesion / Monoclonal antibody / Contact sites A / Dictyostelium discoideum |
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| Research Abstract |
To investigate mutual interactions between purified adhesion protein-impregnated lipid bilayer and intact cells, we prepared 3 sorts of monoclonal antibodies against cell adhesion proteins, 80kDa glycoprotein. 1st antibodies in 3 categories recognized a protein portion(s) of 80 kDa protein. 2nd antibodies recognized type II sugar chain(s) of the glycoproteins because they bound a typeI sugar-deficient glycoprotein derived from mutant HG 220. 3rd antibodies bound type I sugar chain of the glycoproteins because the antibodies bound type II sugar-deficient glycoproteins, which derived from cells with tunicamycin.
Next we tried to introduce cell-adhesion protein into artificaial lipid bilayer. We made a Langmuir trough. Lipid monolayers were spread out the air-water interphase in it. The surface pressure was measured with a Wilhelmey plate made from a Millipore filter and attached to a torsion balance. We are now investigating the interaction between intact cells and impregnated lipid bilayers.
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