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1987 Fiscal Year Final Research Report Summary

Production and Mode of Action of Novel Endoglucanase Disintegrating Native Cellulose

Research Project

Project/Area Number 61560126
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionKyushu University

Principal Investigator

HAYASHIDA Shinsaku  Faculty of Agriculture, Kyushu University, 農学部, 教授 (50038197)

Co-Investigator(Kenkyū-buntansha) YOSHINO Sadazo  Faculty of Agriculture, Kyushu University, 農学部, 助手 (80117291)
OHTA Kazuyoshi  Faculty of Agriculture, Kyushu University, 農学部, 助手 (70112315)
OGATA Seiya  Faculty of Agriculture, Kyushu University, 農学部, 教授 (20038277)
Project Period (FY) 1986 – 1987
KeywordsEndoglucanase / Cellobiohydrolase / <beta>-glucosidase / Aspergillus / Humicola / Geotrichum / アビセル吸着性セルラーゼ
Research Abstract

The Avicel-adsorbable endoglucanase (MW. 128,000) and the Avicel-unadsorbable endoglucanase (MW. 63,000) were purified from the culture filtrates of mutant No. 140 and mutant no.191, using gel filtration and ion exchange chromatography. Both enzymes were satble to varying pH and temperature and could rapidly decrease the viscosity of CM-cellulose. The Avicel-adsorbable endoglucanase showed intensive filter-paper-disintegrating activity and a highly synergistic effect with the cellulase Preparation of Trichoderma reesei in the hydrolysis of microcrystalline cellulose. This endoglucanase was observed via scanning electron microscopy to disintegrate Avicel fibrils layer by layer from the surface, yielding thin sections with exposed chain ends. The Avicel-unadsorbable endoglucanase showed no disintegrating activity on filter paper and Avicel and a less synergistic effect with the T. reesei cellulase in hydrolyzing microcrystalline cellulose than did the former enzyme. Endoglucanase was therefore divided into two types, Avicel-disintegrating and -nondisintegrating.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] R.M.Matanguihan,K.Mo and S.Hayashida: Annual Reports of ICBiotech. 8. 171-182 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Hayashida and K.Mo: Applied and Environmental Microbiology. 51. 1041-1046 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Mo and S.Hayashida: Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Mo and S.Hayashida: Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Hayashida and K.Mo: Applied and Environmental Microbiology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Hayashida;K.Ohta and K.Mo: "Methods in Enzymology,Vol.160,Chapter34 Cellulases of Humicola insolens and Humicola grisea" Academic Press Inc., 10 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Hayashida;K.Ohta and K.Mo: "Methods in Enzymology,Vol.160,Chapter 86 Xylanase of Talaromyces byssochlamydoides" Academic Press Inc., 4 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R. M. Matanguihan, K. Mo and S. Hayashida: "Studies on Cellulolytic Enzymes from a Strain of Penicillium sp." Annual Reports of ICBiotech. 8. 171-182 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Hayashida and K. Mo: "Production and Characteristics of Avicel-Disintegrating Endoglucanase from a Protease-Negative Humicola grisea var. thermoidea Mutant" Applied and Environmental Microbiology. 51. 1041-1046 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Mo and S. Hayashida: "Production and Characteristics of Two Types of Endoglucanase from Geotrichum candidum" Agricultural and Biological Chemistry. submitted.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Mo and S. Hayashida: "Conversion of Geotrichum candidum Endoglucanase I to Endoglucanase II with Limited Proteolysis" Agricultural and Biological Chemistry. submitted.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Hayashida and K. Mo: "Production and Characteristics of Avicel-Digesting and Avicel-non-digesting Cellobiohydrolases from Aspergillus ficum" Applied and Environmental Microbiology. Submitted.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Hayashida, K. Ohta and K. Mo: Cellulases of Humicola insolens and Humicola grisea (Methods in Enzymology, Vol.160). Academic Press Inc., 323-332 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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