1987 Fiscal Year Final Research Report Summary
Production and Mode of Action of Novel Endoglucanase Disintegrating Native Cellulose
| Project/Area Number |
61560126
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
発酵・醸造
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
HAYASHIDA Shinsaku Faculty of Agriculture, Kyushu University, 農学部, 教授 (50038197)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHINO Sadazo Faculty of Agriculture, Kyushu University, 農学部, 助手 (80117291)
OHTA Kazuyoshi Faculty of Agriculture, Kyushu University, 農学部, 助手 (70112315)
OGATA Seiya Faculty of Agriculture, Kyushu University, 農学部, 教授 (20038277)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Keywords | Endoglucanase / Cellobiohydrolase / <beta>-glucosidase / Aspergillus / Humicola / Geotrichum / アビセル吸着性セルラーゼ |
|---|
| Research Abstract |
The Avicel-adsorbable endoglucanase (MW. 128,000) and the Avicel-unadsorbable endoglucanase (MW. 63,000) were purified from the culture filtrates of mutant No. 140 and mutant no.191, using gel filtration and ion exchange chromatography. Both enzymes were satble to varying pH and temperature and could rapidly decrease the viscosity of CM-cellulose. The Avicel-adsorbable endoglucanase showed intensive filter-paper-disintegrating activity and a highly synergistic effect with the cellulase Preparation of Trichoderma reesei in the hydrolysis of microcrystalline cellulose. This endoglucanase was observed via scanning electron microscopy to disintegrate Avicel fibrils layer by layer from the surface, yielding thin sections with exposed chain ends. The Avicel-unadsorbable endoglucanase showed no disintegrating activity on filter paper and Avicel and a less synergistic effect with the T. reesei cellulase in hydrolyzing microcrystalline cellulose than did the former enzyme. Endoglucanase was therefore divided into two types, Avicel-disintegrating and -nondisintegrating.
|
