1987 Fiscal Year Final Research Report Summary

Analysis of Cell-Cell and Cell-matrix Interactions in a coculture system.

Research Project

Project/Area Number	61570018
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	General anatomy (including Histology/Embryology)
Research Institution	KITASATO UNIVERSITY
Principal Investigator	YAMAMOTO N. Kitasato University, School of Nursing, 看護学部, 教授 (10050543)
Co-Investigator(Kenkyū-buntansha)	NAGAYOSHI K. Kitasato University, School of Nursing, 看護学部, 助手 (00189169) HASEGAWA T. Kitasato University, School of Nursing, 看護学部, 講師 (60180858)
Project Period (FY)	1986 – 1987
Keywords	Cell-Cell Interaction / a prolonged Survival of hepatocytes / Coculture system / 可溶性因子
Research Abstract	When parencymal cells were co-cultured with nonparencymal cells they had a prolonged survival rate and showed stable albumin secretion activity. In this study, the cellular interaction examined in this co-culture system and obtained the following results. 1)Hepatocytes survived for more than 2 months and the finestructures maintained as good as an intact cells. 2)Albumin was immunohistochemically localized in almost all of the hepatocytes over the whole culture period. 3)Some fine fibers(extracellular matrix) were accumulated and covered hepatocyte cord. 4)In a model culture system, which put a memmbrane (having pores, 0.45um in diameter) between parenchymal and nonparenchymal cells, hepatocytes also survived for more than 4 weeks. In the case of dialyzing memmbrane (high molecules cannot pass through the membrane) between hepatocytes and fibroblasts, however, hepatocytes died in a week. These results suggested that in co-culture system some diffusble components may play important roles and one of the components was high molecule.